Preparation and evaluation of antioxidative peptide fraction of Mustelus Griseus muscle protein
Inspired by DPPH·scavenging assay, an efficient method had been developed to acquire muscle hydrolysate (AMH) from Mustelus greseus by an orthogonal L9(3)4 test. Under optimal enzymolysis parameters (enzymolysis time 4 h, enzyme dose 1％, enzymolysis temperature 55 ℃ and pH 6), the DPPHscavenging rate of AMH reached to 27.92％. Enzymolysis time and enzyme dose showed significant effects on hydrolysate preparation (ANOVA p<0.05). Based on molecular weight (MW), AMH-Ⅰ (10<MW<5 kDa), AMH-Ⅱ (3<MW<5 kDa), AMH-Ⅲ (1<MW<3 kDa) and AMH-Ⅳ (MW<1 kDa) were fractionated from AMH by ultrafiltration membrane system, and AMH-Ⅳ showed the best antioxidant activity in the four fractions. Purified by ion-exchange and gel filtration chromatography, F21 and F33 subfractions with stronger antioxidant activity were obtained. The DPPHscavenging activities of F21 and F33 at 2.0 mg/mL were 7.08％ and 8.98％, respectively. The RP-HPLC chromatograms of F21 and F33 showed six and three peaks respectively.
Mustelus griseus hydrolysate antioxidant activity peptide fractions
Zhong Rui Li Bin Wang Qi Hong Zhang Jia Hui Ma Shang Gui Deng
School of Food and Pharmacy, Zhejiang Ocean University, Qixiangtai Road 51, Zhoushan 316004,PR China
国际会议
沈阳
英文
343-347
2011-11-22（万方平台首次上网日期，不代表论文的发表时间）