Isolation and Identification of strains with high Chitin Deacetylase activity
Chitosan is an aboundant biopolymer like cellose. To bioprocess them with chitin deacetylase (CDA), the products will be used in medicine and food industry. In order to get new strains with significant ability of (CDA) Twenty eight strains producing CDA were isolated and screened out from the soil samples by color reaction in plate medium. The strain F2-7-3 was screened out from these strains for the highest CDA activity, which can reach more than 250U/mL. The paranitroacetanilide was used as the substrate, the optimum temperature for enzyme activity was determined to be 50℃ and the optimum pH was 7.0. It was studied by method of morphological, physiological and biochemical characteristics and 16S rDNA analysis. Data shows that the strain was Rhodococcus sp.
Chitosan Rhodococcus sp Chitin deacetylase Enzyme active
Gou Jingxuan Dong Wenbin Zeng Qiao Zhang Jingjing
College of Chemistry and Chemical Industry, Shaanxi University of Science and Technology, Xi’an,Shaa College of Life Science and Technology, Shaanxi University of Science and Technology, Xi’an,Shaanxi,
国际会议
沈阳
英文
476-479
2011-11-22（万方平台首次上网日期，不代表论文的发表时间）