Linear fitting study on OD values and the number of two facultative anaerobic live bacteria
With the decrease of conventional energy, the development and utilization of bioethanol became a hot topic. Among many microorganisms that have been exploited for ethanol production, Yeast and Zymomonas mobilis have been intensively studied. The yield and quality of bioethanol directly depended on the activity and bacterial counts of this two, which was measured by the optical density (OD). In this paper, the relationship between OD values, and the number of Yeast and Zymomonas mobilis. After Yeast and Zymomonas mobilis were activated, 6.5mL sterilizated saline were adding to agar slant respectively. Then lml mixed bacterial liquid was diluted to 2.0, 3.4, 3.8,4.2, 4.6 and 5.0ml, in which the counts of Yeast and Zymomonas mobilis were determined with the method of dull colony notation. Two linear regression equations were obtained, one was Y1=0.166X+0.777 (R2= 0.956),whereX1 was the OD460 value, and Y1 was the number of viable cells(× 106 / ml). The other one was Y2= 0.147X2+0.137 (R2=0.984) , where X2 was the OD600 value, and Y2 was the number of viable cells(x 106 / ml). The bacterial counts of Yeast and Zymomonas mobilis can be got quickly and accurately by measuring the OD values, calculating from the two equations above.
bioethanol Yeast zymomonas mobilis OD value
Hailing Ma Xu Wang Mingyue Zheng Mingxia Zheng Honghan Chen
School of Water Resources and Environment Science, China University of Geosciences-Beijing, Beijing Civil & Environmental Engineering School, University of Science & Technology Beijing, Beijing 100083 Department of Environmental Science and Engineering, Tsinghua University,Beijing 100084
国际会议
天津
英文
295-298
2011-11-12（万方平台首次上网日期，不代表论文的发表时间）