Performance and Microbial Community Dynamics in Anaerobic Bioreactor Treating Swine Manure
To investigate the relationship between the change of microbial community structure and the performances of anaerobic digestion bioreactor, a fermentation of swine manure was operated in a completely stirred tank reactor at a mesophilic temperature last for 90 days. The process of reactor can be directly characterized by physico-chemical data, such as gas production, VFA, pH and COD were recorded or analyzed twice a week. Bacteria and archaea were both analyzed using specific primers for nested PCR and their community dynamics were presented by denaturing gradient gel electrophoresis (DGGE). The results showed that the developments of bacteria and archaea were different with each other. The coefficient similarity of bacteria was 45％ between the beginning and the ending, while the archaea was only 15％. In addition, the trend of both bacteria and archaea were highly related to the physico-chemical parameters. Abundance of archaea was increased with a rush after 30 days when the pH was higher than 7.0, which may due to the degradation of VFA. Bacteria were changed dramatically which was associated to the available COD during the whole process. Illustrating the complex evolution and coordination of microbial community may become more useful to monitor the anaerobic digestion bioreactor.
Anaerobic Digestion Microbial Community PCR-DGGE Swine Manure
Shiyou Liu Jianbin Ye Qin Xiao Yaoyu Feng Wei Jin
School of Resource and Environmental Engineering, East China University of Science and Technology, S College of Environmental Science and Engineering, Tongji University, Shanghai,China
国际会议
天津
英文
348-357
2011-11-12（万方平台首次上网日期，不代表论文的发表时间）