Studies of Optimum Conditions for Cross-linking Immobilization of Alkaline Protease on Graphene Oxide
Graphene oxide was used as substrate for immobilizing alkaline protease. The immobilizing conditions were optimized though single-factor test and orthogonal design. Then the activity recovery was raised to 92.0％ and 70.1％ in the optimum conditions of immobilization. The test indicated that the optimum technics were as follows: the enzyme amount to 1 mg carriers was 500(xl, the concentration glutaraldehyde is 1％, pH was 8.0, temperature was 25°C, crosslinking time and absorption were 4 hours and 1 hour, respectively, the recycling rate of activity (RRA) reached 70.1％.
Graphene Oxide Alkaline Protease Immobilization
Ruijing Su Wenfeng Zhang Penghui Shi Mincong Zhu Dengxin Li
College of Environmental Science and Engineering, Donghua University Songjiang District, Shanghai 201620, China
国际会议
大连
英文
15-19
2011-10-19（万方平台首次上网日期，不代表论文的发表时间）