Research on Fluorescence Imaging Detecting System of Intracellular Calcium
A new effective imaging detecting system has been used to determinate of intracellular calcium. The fluorescence Image acquisition and analysis was realized with fluorescence imaging detecting system. A designed microfluidic chip has been built. Cell fostering, staining and other operations can be implemented on it. Based on it, a new detecting means was developed for fluorometric measurement. The concentration of intracellular calcium ion can been calculated by using the fluorescence ratio method. A series of experiments were performed by using the detecting system. The experimental results show the ratio of fluorescence intensity was positively correlated to the raising temperature and enhancing shear stress.
imaging detecting fluorescence intensity the concentration of calcium ion microfluidic chip cell detection fluorescence ratio shear stress
Bao Liu Sixiang Zhang Wei Zhou
Research Institute of Modern Measurement & Control Technology, Hebei University of Technology, Tianjin, China
国际会议
大连
英文
38-41
2011-10-19（万方平台首次上网日期，不代表论文的发表时间）