A LED-induced Confocal Fluorescence Detection System for Quantitative PCR Instruments
Fluorescent quantitative PCR (Q-PCR) technology has been an active research area since it has excellent performance of fluorescence signal detection and the ability of quantitative analysis for initial DNA template evaluation. A compact design of fluorescence detection system based on enhanced confocal type is developed. This paper basically focuses on the features of each component of the system and the determination of device lectotype. Quantified results of the experimental test show a much lower background noise level, and 8-tube scanning of double diluted rhodamine B solutions (minimal concentration: 1.21 ppm) ulteriorly demonstrates the effectiveness of the instrument we designed.
fluorescence detection quantitative PCR system design experimental test
Keke Zhong Zhangwei Chen Jing Huang He Mao Kewei Hu
State Key Laboratory of Fluid Power and Control Zhejiang University Hangzhou, 310027, P.R.China
国际会议
上海
英文
1020-1024
2011-10-15（万方平台首次上网日期，不代表论文的发表时间）