Cell Cytotoxicity Analysis of Adherent and Suspended Cancer Cells Using an Integrated Microfluidic Device
In this paper, a multilayer elastomeric microfluidic device with micro cell sieves was developed for an assay of drug induced cytotoxicity. We evaluated the cytotoxic effects of anti-cancer drugs tamoxifen (TAM) and camptothecin using MDA-MB-231 breast cancer cells and human chronic myeloid leukaemia K562 cells respectively. To validate cell morphology and viability on the microfluidic chip, fluorescent cell viability assay was carried out using three fluorescent dyes. The results showed that TAM and camptothecin both induced cytotoxicity of the two cell types in a concentration-dependent manner. Using this scalable platform, cell seeding and anti-cancer drugs exposure can be carried out within a single microfluidic device in a multiplexed format, enabling high-density parallel cytotoxicity screening while minimizing reagent consumption.
microfluidic device MDA-MB-231 cells KS62 cells anti-cancer drugs cytotoxicity
Huixue Song Shaotie Peng Tan Chen Baoyue Zhang Zhanhui Wang
Key Lab of Health Informatics of Chinese Academy of Sciences, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences,Shenzhen, P.R. China 518055
国际会议
上海
英文
1057-1060
2011-10-15（万方平台首次上网日期，不代表论文的发表时间）