Design of Signal Preamplifier Circuit for Fluorescence Detection System in Quantitative PCR Instrument

摘要：

Quantitative Polymerase Chain Reaction (qPCR) has revolutionized the detection of DNA and RNA. But the quantification results are susceptible to the effects of background noise and the intensity of detected fluorescent signals. Aiming at solving the above problem, this paper presents a signal preamplifier circuit which is compatible to the fluorescence detection system. In the entire process of genetic quantification, the signal preamplifier circuit plays a very important role in amplifying the subtle fluorescent signal and eliminating the noise in the meantime. Experiment results show that with the help of this amplifier, the qPCR instrument can have a better performance in detecting weak fluorescent signals and precise quantification.

关键词： qPCR fluorescence detection system amplifier circuit, noise suppression PMT

作者: He Mao Zhangwei Chen Jing Huang Keke Zhong Kewei Hu

作者单位: Department of Mechanical Engineering, Zhejiang University,Hangzhou 310027, China

会议类型: 国际会议

会议名称: 2011 4th International Conference on Biomedical Engineering and Informatics(第四届生物医学工程与信息学国际会议 BMEI 2011)

会议地点: 上海

会议语种:英文

页码: 1083-1086

在线出版日期: 2011-10-15（万方平台首次上网日期，不代表论文的发表时间）