Research on Temperature Measuring Positions Selection and Verification for Polymerase Chain Reaction Instruments
Specific fragments of DNA in vitro can be rapidly amplified after several thermal cycles of the test samples by taking Polymerase Chain Reaction technology. The good performance of temperature control in thermal cycles is critical to successful PCR reaction. However, in order to achieve good performance of temperature control and guarantee the temperature qualities of PCR instruments, it is crucial to precisely measure the temperature field of sample block by selecting appropriate temperature measuring positions on the sample block. Simulation in finite element analysis (FEA) is carried out by using software ABAQUS 6.9 in this paper in order to select appropriate temperature measuring positions on the sample block, meanwhile, the experiment concerned is conducted . The experimental results indicate that the temperature field distribution of sample block is able to be fully represented and illustrated through 13 selected temperature measuring positions.
PCR temperature measuring position FEA experiment
Kewei Hu Zhangwei Chen Jing Huang
State Key Laboratory of Fluid Power and Control Zhejiang University Hangzhou, 310027, P.R. China
国际会议
上海
英文
1171-1175
2011-10-15（万方平台首次上网日期，不代表论文的发表时间）