Differential SHH and IHH Expression during Osteogenesis Induced by Dexamethasone in Rat Bone Marrow Mesenchymal Cells
Hedgehog signaling pathway is an important signal pathway, which regulates the development of variety of tissues and organs in the embryonic stage. It also plays an important role in osteogenesis. However, there are few data recording the role of Hedgehog signaling molecules in osteogenic differentiation of bone marrow mesenchymal stem cells in vitro. Dexamethasone (DEX), a synthetic and widely used glucocorticoid, affects osteogenesis. And the signaling pathway by which DEX affects osteoblastic differentiation remains obscure. Here to investigate the expressions of Hedgehog signaling molecules, Sonic Hedgehog (SHH) and Indian Hedgehog (IHH), during osteogenic differentiation in rat bone marrow mesenchymal stem cells (MSCs) induced by Dexamethasone. In this study, we found that SHH expression significantly increased while IHH expression decreased during osteoblastic differentiation of MSCs induced by Dexamethasone at the concentration of 10 -7M from 7 days to 21 days. These findings suggest that Hedgehog signaling molecules, SHH and IHH, involve in the osteogenic differentiation of bone marrow stromal cells induced by Dexamethasone with various effects. To further study the mechanism of the role of Hh signaling molecules on osteogenic differentiation in MSCs would provide new data for bone tissue engineering.
Sonic Hedgehog Indian Hedgehog Osteogenic Differentiation Mesenchymal Stem Cells
Xiaoli Ma Haiji Sun Liguo An Shanshan Zu Yanfei Jia Yan Zheng Shuyi Han Hua Liu Dongjie Xiao Yunshan Wang Xueping Zhang
Medical Research and Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong Uni College of Life Science, Shandong Normal University, Jinan 250014, China Medical Research and Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong Uni
国际会议
上海
英文
1211-1214
2011-10-15（万方平台首次上网日期，不代表论文的发表时间）