Experimental Investigation of Temperature-Related Changes in Viscoelastic Properties of Articular Chondrocytes
in our previous investigations, we have proved that the viscoelasticity of the Articular chondrocytes undergoes dramatic changes during their normal development, however, whether the temperature, one of the important factors in cell cultivation and cartilage tissue engineering, is related with the viscoelasticity of cell in not known. The goal of this study was to characterize the changes of viscoelastic properties of chondrocytes in different age group under different temperature. The micropipette aspiration combined with a standard linear viscoelastic solid model was used to quantify the effect of temperature changes on viscoelastic properties and deformation recovery of chondrocytes isolated from different age groups. Results have shown that temperature significantly influences the viscoelastic properties of chondrocytes isolated from different group and the deformation recovery of which have significantly changed with temperature changing. Moreover, we found that the cytoskeletal network were more sparsely, and the contents of the components were reduced in old cells determined by immunohistochemistry, LSCM, real time PCR and western blotting compared with the adult and the young cells. These findings provide substantial temperaturerelated information which may be relevant for chondrocyte-based cartilage tissue engineering.
age-related temperature chondrocyte viscoelastic property micropipette aspiration
Zhang Quanyou Liu Linlin Li Chunjiang Duan Wangping
Department of Engineering Mechanics, College of Sciences. Taiyuan University of Technology Taiyuan, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan Department of Orthopaedics, Second Hospital of Shanxi Medical University, Taiyuan, China, 030001
国际会议
上海
英文
1225-1228
2011-10-15（万方平台首次上网日期，不代表论文的发表时间）