Development and primary application of the S YBR Green I RT-qPCR Assay for the detection of the Transmissible Gastroenteritis Virus (TGEV) S gene

摘要：

This study established a SYBR Green I real-time quantitative PCR detection assay (RT-qPCR) for the detection of the porcine transmissible gastroenteritis virus (TGEV) using specific primers designed to amplify the highly conserved porcine TGEV S gene sequence. The threshold cycle (Ct) and the log plasmid copy numbers had a good linear relationship with an efficiency of 1.05 (R2 = 0.999). The advantages of utilizing this approach for the rapid detection of TGEV include excellent sensitivity, reproducibility, and low cost. Ninety-six porcine fecal samples were tested in this study, and 7 samples more than PCR assay were detected by this assay.

关键词： TGEV SYBR Green I RT-qPCR S gene

作者: Jiangping Gu Xiuwei Yue Congli Yuan Li Cui Xiuguo Hua Zhibiao Yang Caiying Li Rui Xing

作者单位: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jia Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jia

会议类型: 国际会议

会议名称: 2011 4th International Conference on Biomedical Engineering and Informatics(第四届生物医学工程与信息学国际会议 BMEI 2011)

会议地点: 上海

会议语种:英文

页码: 1423-1426

在线出版日期: 2011-10-15（万方平台首次上网日期，不代表论文的发表时间）