Relationship between Protective Effect of Hesperidin Extraction on Exercise Fatigue and Antioxidation
Objective: To explore the antioxidative effect of hesperidin extracted from the peel of Gannan kumquat on exercise fatigue. Methods: Hesperidin was extracted from the peel of kumquat by ultrasound and ethanol precipitation and was detected qualitatively by the high performance liquid chromatogramphy. Animal model of exercise fatigue was established by the 10-day increasing progressively swimming training. Effect of hesperidin on serum glutathione peroxidase, superoxide dismutase, catalase, myeloperoxidase and maleic dialdehyde of exercisefatigue rats was tested by the spectrophotometric method. Results: The rats of exercise fatigue had some phenomena, such as depilation, tired appearance, low appetite, degressive locomotory capacity and slow-moving growth of body weight Exercise fatigue caused the rats to decrease the enzyme activity of serum GSH-PX (P<0.001) and to increase the enzyme activity of serum MPO (P<0.05); however, exercise fatigue had no significant difference on the enzyme activity of serum SOD and CAT, and concentration of MDA (P>0.05). The rats of intragastric administration by extractive hesperidin had fine appetite, glossy hair, persistent growth of body weight. Hesperidin had no positive and negative function on the enzyme activity of serum SOD and CAT of exercise fatigue rats (F=0.4589, P>0.05; F=0.8556, P>0.05). Low, middle and high dose of hesperidin had no influence on the enzyme activity of serum MPO (P>0.05); nevertheless, low, middle and high dose of hesperidin increased the enzyme activity of serum GSH-PX (P< 0.001) although it couldnt recover the normal level of enzyme activity of serum GSH-PX. At the same time, hesperidin decreased the concentration of serum MDA in exercise fatigue rats (P<0.001). Conclusion: Hesperidin had inhibited the exercise fatigue through increasing the enzyme activity of serum GSH-PX, decreasing the concentration of serum MDA.
hesperidin exercise fatigue antioxidation
Xiaoting Luo Shumei Li Sisi Li Liang Xiong Minghua Dong
Department of Biochemistry and Molecular Biology Department of Preventive Medicine Gannan Medical College Ganzhou, China
国际会议
上海
英文
1450-1453
2011-10-15（万方平台首次上网日期，不代表论文的发表时间）