The Effects of mechanical stimulation on proliferation and CTGF expression of human lung fibroblast

摘要：

To understand the effect of mechanical stimulation on proliferation and connective tissue growth factor (CTGF) expression of human lung fibroblast (MRC-5), the cells were subjected to cyclic stretch regimens (sine wave, 5％ and 10％ elongation amplitude, 0.2Hz, 48h duration) by FX-4000 Tension System. The MRC-5 proliferation was examined and analyzed by flow cytometry following 48h of stretch, the CTGF expression was detected by ELISA method following 12h, 24h, 36h, and 48h of stretch. Results: the 5％ stretch promoted the proliferate activity and did not affect the CTGF expression of MRC-5; however, the 10％ stretch inhibited the proliferation of MRC-5 and increased the CTGF expression at 36h and 48h (P <0.05). It is concluded that mechanical stimulation play important role in the proliferation activity and CTGF expression of lung fibroblast.

关键词： lung fibroblast mechanical stimulation CTGF proliferation

作者: Guohui Wang Yongpeng Xie Dan Shi Nan Zhang

作者单位: College of pharmacy and Biological Science Weifang Medical University Weifang, China Emergency department Lianyungang first peoples hospital Lianyungang, China Emergency department Changchun university hospital of traditional Chinese medicine Changchun, China Emergency department The first hospital of Jilin university Changchun, China

会议类型: 国际会议

会议名称: 2011 4th International Conference on Biomedical Engineering and Informatics(第四届生物医学工程与信息学国际会议 BMEI 2011)

会议地点: 上海

会议语种:英文

页码: 1549-1552

在线出版日期: 2011-10-15（万方平台首次上网日期，不代表论文的发表时间）