会议专题

Isolation and identification of Vibrio rotiferianus from diseased half-smooth tongue sole (Cynoglossus semilaevis Gunther)

Four strains dominant bacteria associated with skin ulcers were isolated from half-smooth tongue sole, Cynoglossus semilaevis. Bacteria strain BV1 was proved to be a pathogen by muscle injection with bacteria suspension, the LD50 was 1.5×105 cfu/mL. The morphological, physiological and biochemical characteristics were examined, the 16S rRNA and gyrB gene were partially sequenced and compared with sequences deposited in genebank, and the molecular phylogenetic trees were constructed. The sequenced 16S rRNA gene of strain BV1 (GenBank accession No. JN391272) is 1379bp in length, the sequenced gyrB gene of strain BV1 (GenBank accession No. JN 168882) is 1130bp in length, and gyrB gene exhibited high similarity in 99% with the gyrB gene of Vibrio rotiferianus from GenBank database. The results of physiological and biochemical tests and molecular identification suggested that the pathogen was V. rotiferianus. Detection of the activity of extracellular products (ECP) and hemolysin showed that the strain BV1 could produced amylase, lipase, urease, lecithinase, and with β-haemolysis in containing 4% defibrinated rabbit blood of TSA plates, but no proteinase, gelatinase. Drug resistance of the pathogen to 17 antimicrobial agents were detected, the results showed that the strain was sensitive to streptomycin, tetracycline and so on.

Cynoglossus semilaevis Vibrio rotiferianus 16S rRNA gene gyrB gene extracellular proucts

Zhengqiang Chen Zhixian Yao Mao Lin Jianbo Chang

Fisheries College Jimei University Xiamen, Fujian 361021, China

国际会议

2011 4th International Conference on Biomedical Engineering and Informatics(第四届生物医学工程与信息学国际会议 BMEI 2011)

上海

英文

2253-2258

2011-10-15(万方平台首次上网日期,不代表论文的发表时间)