The effects and mechanism of down-regulation of survivin expression by RNAinterference on the proliferation and apoptosis of lung cancer cell
Survivin, a member of inhibitor of apoptosis family protein, has become an attractive therapeutic target in cancer due to its selective expression in tumor cells and its important roles for tumor cell viability. The aim of this study was to investigate the effect of the survivin-siRNA plasmid on survivin expression in the human lung cancer cell line A549, and to observe its effect on the apoptosis and proliferation of A549 cells. Human lung cancer cell line A549 was transfected with survivin -targeted small interfering RNA (siRNA). The down-regulation of survivin expression was determined by real-time polymerase chain reaction and Western blot. The proliferation of A549 cells was determined by MTT assay. The apoptosis rate and cell cycle distribution were analyzed by flow cytometry (FCM). Caspase-9 activity was also detected to study the apoptosis of lung cancer cells induced by survivin-siRNA. The sequence-specific siRNA efficiently and specifically down-regulated the expression of survivin at both mRNA and protein levels. Down-regulation of survivin expression dramatically suppressed the proliferation of A549 cells, and arrested the cells at G (1)/G (0) phase. Caspase-9 activity was significantly increased in A549 cells transfected with survivin-siRNA. hi this study, we found that Survivin-specific siRNA.
RNA interference Survivin Lung cancer Proliferation Apoptosis
XIANG-QI CHEN SHENG YANG ZHI-YING LI HUI-SHAN LU MING-QIANG KANG TING-YAN LIN
Department of Respiratory, the Union Hospital of Fu Jian Medical University, Fuzhou, 350001, P.R. Ch Department of Oncology, the Union Hospital of Fu Jian Medical University, Fuzhou, 350001, P. R.China Department of Thoracic Surgery, the Union Hospital of Fu Jian Medical University, Fuzhou,350001, P.
国际会议
亚太地区国际肿瘤生物学和医学学术会议、全国肿瘤标志学术大会、第六届中国中青年肿瘤专家论坛
上海
英文
127-136
2011-10-13（万方平台首次上网日期，不代表论文的发表时间）