Expression of survivin in human lung cancer and the influence of its down-regulation on biological behavior of this cancer
The aim of our study was to detect the expression of survivin in human lung cancer and the influence of its down-regulation on biological behavior of lung cancer A549 cell. The high expression of survivin in human lung cancer was verified by immunohistochemistry (IHC) method. Survivin-siRNA (small interfering RNA) and unrelated sequence were synthesized and siRNA lentiviral vector was then constructed. The vector was then transfected into lung cancer A549 cell, in which the clone with stable expression was screened out. We blocked the expression of survivin mRNA and protein by RNA interference (RNAi) technique. The down-regulation of survivin mRNA and protein expression was determined by real-time quantitative PCR (RT-qPCR) and Western blot. The proliferating activity and growth speed of A549 cell were determined by colony formation assay (CFA) and mono-nuclear cell direct cytotoxicity assay (MTT Assay). The GBM penetrating capacity was determined by cell invasion assay. The cell movement and migration capacity were detected by erasion trace test. The results showed that the sequence-specific siRNA significantly down-regulated the expression of survivin at both mRNA and protein levels. Down regulation of survivin expression dramatically decreased the invasive and metastatic capacities of the cell, suppressed the proliferation, decelerated the speed of growth, reduced the number of clones on soft agar and decreased the capacity of GBM penetrating and migration. In conclusion, Survivin, which played an important role in carcinogenesis and development of lung cancer, could be effectively down-regulated by RNAi technique.
survivin RNA interference Lung cancer biological behavior
Sheng Yang Xiang-qi Chen Zhi-ying Li Hui-shan Lu Ting-yan Lin Ming-qiang Kang
Department of Oncology, the Union Hospital of Fu Jian Medical University, Fuzhou, 350001, P. R.China Department of Respiratory, the Union Hospital of Fu Jian Medical University, Fuzhou, 350001, P.R. Ch Department of Thoracic Surgery, the Union Hospital of Fu Jian Medical University, Fuzhou,350001, P.
国际会议
亚太地区国际肿瘤生物学和医学学术会议、全国肿瘤标志学术大会、第六届中国中青年肿瘤专家论坛
上海
英文
137-146
2011-10-13（万方平台首次上网日期，不代表论文的发表时间）