Upregulation of DNA polymerase iota (Pol ι) in esophageal squamous cell carcinoma

摘要：

The transcriptional regulation of low-fidelity translesion DNA synthesis (TLS) polymerases in esophageal cancer was investigated. We found significant overexpression of mRNA levels for polymerase zeta (Pol ?), RAD18, polymerase iota (Pol i) and polymerase kappa (Pol k) in esophageal carcinomas. Pol t was further studied in greater detail. The promoter of POLI, encoding Pol x, was hypomethylated and its expression was not regulated by methylation. Spl and Oct-1 binding sites were identified in the POLI promoter. POLI transcription was correlated with that of Spl, and cancerous tissues showed significantly higher Spl (~4-fold) but not Oct-1 binding affinity, relative to nontumor samples. Taken together, our data presented the expression profile of TLS genes in esophageal cancer and elucidated the mechanism for POLI upregulation.

关键词： esophageal squamous cell carcinoma (ESCC) translesion DNA synthesis (TLS) polymerase iota (Pol i) promoter Sp1

作者: Jundong Zhou Shuyu Zhang Xie lin Pengfei Liu Fang Xie Xiaozhong Zhu Jianping Cao Jinchang Wu

作者单位: School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123 School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123 Department of Radiotherapy of Suzhou Municipal Hospital, Suzhou 215001, China Department of Gastroenterology, the Affiliated Jiangyin Hospital of Southeast University, Jiangyin 2 The State Key Laboratory of Genetic Engineering and School of Life Science, Fudan University, Shangh Department of Thoracic Surgery, the Affiliated Hospital of Jiangsu University,Zheniiang 212001, Chin

会议类型: 国际会议

会议名称: 亚太地区国际肿瘤生物学和医学学术会议、全国肿瘤标志学术大会、第六届中国中青年肿瘤专家论坛

会议地点: 上海

会议语种:英文

页码: 269-277

在线出版日期: 2011-10-13（万方平台首次上网日期，不代表论文的发表时间）