PERIPHERAL BLOOD MRNA EXPRESSION PATTERNS TO DIFFERENTIATE HEPATOCELLULAR CARCINOMA FROM OTHER HEPATIC DISEASES
(0) Objectives:Peripheral blood genes expressions profiling (GeXP) have been convinced to be more specific for the diagnosis of cancer and other diseases, and the GeXP system provides an ideal method to analyze multiple genes expression in one normalized and equable system. We aim to differentiate hepatocellular carcinoma from other hepatic diseases based on peripheral blood and the GeXP system. Methods:Fifteen selected hepatic diseases related genes with two house-keeping genes for normalization were detected by the GeXP system. The diagnosis model was based on K nearest neighbor classifier and cross validation, and software based on MAFLAB software was built for differential diagnosis of hepatic diseases. Results:Eight hepatic related genes were demonstrated to show an obvious statistic difference in expressions while the K nearest neighbors classifier showed that the accuracy for normal controls, hepatitis B, liver cirrhosis, hepatocellular carcinoma and the Other group was separately 80.57％, 78.17％, 84.48％, 73.24％ and 85.85％. The set of validation has been carried out to assess the accuracy of Model Two and the accuracy was even higher than the set of building for the model, except for the hepatitis B (HBV) group.Conclusion: A sensitive and specific GeXP system of eight genes has been developed for the accurate differential diagnosis of hepatic disease.
Peripheral blood GeXP Hepatocellular carcinoma Cross validation Genes expression
Zhang P J Dong ZN Zhang XJ Tian Y P Run W Liang P Wang C B Deng X X Wang B Chen B Jiao J Liu H Y
Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, China Department of Interventiont Ultrasound, Chinese PLA General Hospital, Beijing, China Department of Clinical Laboratory Medicine, Chinese PLA General Hospital, Beijing, China
国际会议
亚太地区国际肿瘤生物学和医学学术会议、全国肿瘤标志学术大会、第六届中国中青年肿瘤专家论坛
上海
英文
290-306
2011-10-13（万方平台首次上网日期，不代表论文的发表时间）