Single Nudeotide Polymorphisms in the CDH17 Gene (cJ43A>G and C.2216A > C) of Colorectal Carcinoma in Chinese Han Population of Henan Province
AIMS: To investigate the relationship between the single nucleotide polymorphisms (SNPs) of C.343A > G and C.2216A > C polymorphism sites in the liverintestine cadherin (CDH17) gene and susceptibility to colorectal carcinoma. METHODS: The SNPs of the CDH17 gene C.343A > G and C.2216A > C were determined by the polymerase chain reaction-single strand conformation polymorphism method (PCR-SSCP) in 93 peripheral venous blood samples from colorectal carcinoma patients. Typical samples that showed different migration bands in SSCP were confirmed by sequencing. RESULTS: There is a significant association between the c.2216 A > C SNPs of CDH17 gene and TNM grade as well as with lymph node status in 93 peripheral venous blood samples from colorectal carcinoma patients. The genotype frequencies of A/C, A/A and C/C were 12.90％, 33.33％ and 53.76％, respectively. There were a significant correlation between lymph node metastasis and TNM grade and the genotype distribution (P<0.05). The C/C genotype raised risk of lymph node metastasis (OR of genotype C/C, genotype A/A to genotype A/C were 5.417 and 1.200, respectively). And there was a significant difference in lymph node metastasis between the A/A and C/C genotypes (P=0.003). Patients with colorectal carcinoma carrying the C allele tended to have a higher risk of lymph node metastasis (OR of allele C to allele A was 3.990). And thedifference between the lymph node metastasis of two alleles was statistically significant (χ2=16.488; df=l;P=0.000). In addition, the genotype C/C raised risk of TNM grade (the mean ranks of A/A, A/C and C/C genotype were 38.77,42.00 and 53.30, respectively). And there was a significant difference in TNM grade between the A/A and C/C genotypes (P=0.013). Patients with colorectal carcinoma carrying the C allele tended to have a higher TNM grade (die mean ranks of allele A and allele C were 78.09 and 103.68, respectively). And the difference between the TNM grade of two alleles was statistically significant (H=11.225; df=1; p=0.001). CONCLUSION: The SNPs of CDH17 gene c.2216 A >C might be clinically important in prognosis of colorectal carcinoma.
colorectal carcinoma CDH17 gene the single nucleotide polymorphisms
Juan-Juan Cao Juan Chen Jian-Ping Yang Xiao-Bo Liu Guo-Qiang Zhao Yu-Feng Zhang Ren-Yin Chen
Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China 450 Zhengzhou University School of pharmaceutical Sciences 450001 Basic Medical College of ZhengZhou University 450001 Department of Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China 45005
国际会议
亚太地区国际肿瘤生物学和医学学术会议、全国肿瘤标志学术大会、第六届中国中青年肿瘤专家论坛
上海
英文
343-368
2011-10-13（万方平台首次上网日期，不代表论文的发表时间）