A pre-column derivatization-HPLC method for determination of oxalipIatin(L-OHP)content and entrapment efficiency in nanoparticles
Purpose: To develop an improved -HPLC method for the determination of oxaliplatin(L-OHP) content and entrapment efficiency in nanoparticles. Methods: After derivatization with sodium diethyldithiocarbamate (DDTC), Pt(DDTC)2 and diazepam (the internal standard) was separated on C18 column (250 mm×4.6 mm, 5 urn) at 30°Cusing a mobile phase consisting of methol and water (75:25, v/v) at flow rate of 1 ml-min-1; The method was validated with respect to selectivity, linearity, precision and accuracy, limit of quantification and stability. The ultrafiltration was employed to separate the free L-OHP from nanoparticles to calculate the entrapment efficiency. Results: The calibration curve of L-OHP was linear in the concentration range of 5.26-31.53 ugml-1. (r= 0.9997) with the limit of quantification (LOQ) of 0.42 ug/mL. The variation coefficients were not more than 1.63％. The mean recoveries was from 98.29％ to 100.45％. The content and the encapsulation efficiency of L-OHP nanoparticles were 1.69mg/ml and 63％, respectively. Conclusion:The present method can be used to determine L-OHP content in prepared nanoparticles.as well as entrapment efficiency.
Oxaliplatin Pre-column derivatization HPLC Nanoparticles Entrapment efficiency
Xiaodan Zhou Li Yang Shu Wang Xiaojiao Tang Qi Zhai
Department of pharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, PR China
国际会议
The Second Asian Symposium on Pharmaceutical Sciences and Technology(第二届亚洲药物制剂科学研讨会)
西安
英文
101-102
2011-09-19（万方平台首次上网日期，不代表论文的发表时间）