Research on the microbial shifting of marmatite bioleaching
The microbial community shifts that occur during marmatite bioleaching are poorly understood and little effort has been made to characterize the microbiological components of this process. A mixed culture of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Acidithiobacillus caldus was applied to bioleaching of marmatite in this study. Scanning electron microscopy (SEM) , Xray diffraction (XRD) and energy dispersive X-ray analysis ( EDX) observations showed compositional changes, surface features of the leaching residues and biofilm formation on marmatite. 16S ribosomal deoxyribonucleic acid ( rDNA) -based denaturing gradient gel electrophoresis ( DGGE) was applied to track the succession of microbial communities in marmatite bioleaching, which identified significant changes in species composition. Multidimensional scaling ( MDS) analysis of DGGE banding patterns revealed that the marmatite bioleaching process could be divided into 3 stages at 30℃ and 2 stages at 35℃. Results outlined in this work extend our knowledge of microbial dynamics in marmatite bioleaching, therefore improving its commercial applications.
Acidithiobacillus ferrooxidans Acidithiobacillus thiooxidans Acidithiobacillus caldus marmatite bioleaching
Zhiguo He Shuzhen Li Fengling Gao Guohua Gu Yuehua Hu Guanzhou Qiu
School of Minerals Processing and Bioengineering, Central South University,No. 932,South Lushan Road, 410083, Changsha, China Key Laboratory of Biometallurgy, Ministry of Education No. 932,South Lushan Road, 410083, Changsha, China These authors contributed equally to this work
国际会议
The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)
长沙
英文
266-273
2011-09-18（万方平台首次上网日期，不代表论文的发表时间）