Effect of pH and temperature on the adsorption of Acidithiobacillus caldus, Leptospirillum ferriphilum and Sulfobacillus thermosulfidooxidans onto pyrite
The moderately thermophiles A. c, L.f and S. t were mainly bioleaching bacteria. Attachment to mineral surfaces enhanced bioleaching pyrite by these bacteria. Effect of different temperatures (40℃ and 45°C ) and pH values (1.6, 2. 0 and 2. 5) on the adhesion of bacterial cells onto pyrite surface was investigated. At 40℃ , L.f exhibited the highest adsorption rate of 89.47％ at pH 2.0, and mixed culture (A. c, L. f and S. t) with an adsorption rate of 75. 54％ at pH 1.6. The contact angles and zeta potentials of pyrite-grown bacteria were found to be higher than those of Fe-grown and sulfur-grown bacteria. The sessile cells on the pyrite surfaces were found in single cells or in clusters visualized after DAPI staining by EFM. And pyrite surface was found to be severely corroded after interaction with 5. t, which showed that 5. t had stronger oxidizing ability to pyrite than other pure cultures or mixed culture.
bioleaching bacteria extracellular polymeric substances (EPS) energy-dispersive X-ray analysis (EDXA) scanning electron microscope (SEM)
Lisha Wang Jianhua Qu Yuehua Hu Zhiguo He Guohua Gu
School of Minerals Processing and Bioengineering, 410083, Changsha, China Key Laboratory of Biometallurgy of Ministry of Education, Central South University, 410083, Changsha, China
国际会议
The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)
长沙
英文
274-279
2011-09-18（万方平台首次上网日期，不代表论文的发表时间）