13C NMR study on glucose metabolic pathways of genetic engineered chemolithotrophic Acidithiobacillus thiooxidans expressing phosphofructokinase

摘要：

Phosphofructokinase is over expressed in the chemoautotrophic bacterium Acidithiobacillus thiooxidans Tt - 7 ( PSDK - 1). The metabolism pathways of 13 C enriched glucose in recombinant Acidithiobacillus thiooxidans Tt - 7 ( PSDK - 1) were investigated by using 13 C NMR. Glucose metabolism network was constructed to elucidate the distinctive carbon metabolism pathways in the gene engineered chemolithotrophic bacterium. The engineered strain is able to utilize EmbdenMeyerhof ( EM) pathway and the incomplete tricarboxylic acid ( TCA) cycle to convert glucose into a series of intermetabolites, for example, amino acids.

关键词： Acidithiobacillus thiooxidans 13C:NMR, glucose metabolism bioleaching

作者: Wei Liu Jianqun Lin Xiangmei Liu Jianqiang Lin

作者单位: State Key Laboratory of Microbial Technology,Shandong University, 250100, Jinan, China

会议类型: 国际会议

会议名称: The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)

会议地点: 长沙

会议语种:英文

页码: 373-377

在线出版日期: 2011-09-18（万方平台首次上网日期，不代表论文的发表时间）