Increases of ferrous iron oxidation activity and arsenic stressed cell growth by overexpression of Cyc2 in Acidithiobacillus ferrooxidans ATCC19859

摘要：

Acidithiobacillus ferrooxidans plays an important role in bioleaching in reproducing the oxidant of minerals by oxidizing ferrous iron ( Fe2 + ) into ferric iron ( Fe3 + ). In order to increase ferrous iron oxidation activity, CYC2 is over expressed in Acidithiobacillus ferrooxidans ATCC19859. A recombinant plasmid pTCYC2 containing a 6 x His tag fused cycl gene under the control of Ptac promoter is constructed on the basis of the wide-host range plasmid pJRD215 , and transferred into Acidithiobacillus ferrooxidans ATCC19859 using conjugation gene transfer method, cycl gene transcription level was increased by 2. 63 fold in Acidithiobacillus ferrooxidans ( pTCYC2) compared with the control of the wild type strain measured by quantitative real-time PCR. The ferrous iron oxidation activity and the arsenic stressed cell growth of Acidithiobacillus ferrooxidans ( pTCYC2 ) were increased.

关键词： Acidithiobacillus ferrooxidans cyc2 conjugation bioleaching

作者: Wei Liu Jianqun Lin Xin Pang Shuang Mi Shuang Cui Jianqiang Lin

作者单位: State Key Laboratory of Microbial Technology,Shandong University, 250100, Jinan, China

会议类型: 国际会议

会议名称: The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)

会议地点: 长沙

会议语种:英文

页码: 383-387

在线出版日期: 2011-09-18（万方平台首次上网日期，不代表论文的发表时间）