Copper resistance determinants of the biomining thermoacidophilic archaeon Sulfolobus metallicus
Sulfolobus metallicus is a thermoacidophilic archaeon involved in high temperature bioleaching processes that is able to grow under stressing conditions such as high temperature, low pH and high concentrations of heavy metals. S. metallicus tolerates up to 200 mM of copper sulfate ( Cu) compared with 1 mM levels in other species of the Sulfolobus genus. Nevertheless, the genetic and biochemical mechanisms responsible for metal resistance in biomining thermoacidophilic microorganisms remain uncharacterized. In the present report, the presence and co-transcription of duplicated putative genes encoding for Cu-ATPases CopAl and CopA2 and two putative genes for metallochaperones CopMl and CopM2 were characterized in S. metallicus. In addition, by using a proteomic approach, it was found that around 30 proteins were upregulated in S. metallicus cells exposed to 100 mM Cu. Eighteen of these proteins were identified by mass spectrometry, as being related to the production and transport of energy, biosynthesis of amino acids and stress responses. The functional spectrum of upregulated proteins is consistent with other proteomic reports concerned with heavy metals resistance in other prokaryotes and in the acidophilic archaeon Ferroplasma acidarmanus. Finally, this study constitutes the first characterization of Curesistance determinants in a thermophilic archaeon involved in industrial biomining processes.
thermoacidophilic Sulfolobus jnetallicus copper resistance stress response proteomics
Francisco Remonsellez Alvaro Orell Carlos A. Jerez
Laboratory of Molecular Microbiology and Biotechnology, Department of Biology and Millennium Institu Laboratory of Molecular Microbiology and Biotechnology, Department of Biology and Millennium Institu
国际会议
The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)
长沙
英文
388-392
2011-09-18（万方平台首次上网日期，不代表论文的发表时间）