Bioinformatic prediction and experimental verification of sulfate assimilation genes in the extreme acidophile Acidithiobacillus ferrooxidans

摘要：

The sulfate assimilation pathway present in photosynthetic organism, fungi, and many bacteria, provide reduced sulfur for the synthesis of cysteine and methionine and a range of other metabolites. In this paper, we introduced metabolic route for sulfate assimilation in A.ferrooxdians. We listed candidate gene in number 11 open reading frame in this route, which contained cysD-1, cysD-2 , cysN, cysNCl , cysNC2 , cysli , cysG, cys, cys, cysE , cysM,and so on. Expression, characterization and enzymatic analysis of sulfate assimilation pathway involves ATP Sulfrylase, APS reductase, sulfite reductase, serine acetyltransferase, and O-acetylserine sulfhydrylase. We conclude the proposed putative cysteine synthesis pathway in A. ferrooxidans ATCC 23270.

关键词： Acidithiobacillus ferrooxidans cysteine cloning expression

作者: Chunli Zheng Jianshe Liu Min Wang Yunjie Dai

作者单位: College of Environmental Science and Engineering, Donghua University, 201620, Shanghai, China School College of Environmental Science and Engineering, Donghua University, 201620, Shanghai, China School of Resource Processing and Bioengineering, Central South University, 410083, Changsha, China

会议类型: 国际会议

会议名称: The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)

会议地点: 长沙

会议语种:英文

页码: 400-403

在线出版日期: 2011-09-18（万方平台首次上网日期，不代表论文的发表时间）