会议专题

Electrostatic contributions in the adhesion of Escherichia coli and Bacillus subtilis onto gold substrate in aqueous environment

An initial adhesion of microorganisms on gold substrate was investigated using Escherichia coli and Bacillus subtilis in aqueous environment. The influence of electrokinetic parameters like surface potential and energy of adhesion were used to explain the adhesion responses of these microorganisms onto a gold substrate. The electrophoretic mobilities of gold particles and two bacteria were measured using microelectrophoresis method. The zeta potentials were obtained using the Henry-Smoluchowski formula and potential energy curves were determined using the DLVO theory of heterocoagulation. The gold and E. coli exhibited negative surface potentials under all pH conditions while B. subtilis had its iso-electric point (IEP) at pH 3. 9. The growth of the two bacteria in KNO3, as the supporting electrolyte, was also monitored and growth curves were generated. Actual adhesion is done separately on the two bacteria using a gold plate. After adhesion runs, the number of adhered bacteria is quantified using serial dilution and spread plating to obtain CFUs to determine the number of live bacteria that adhered onto the gold plate. It was observed that in varying ionic concentration, E. coli exhibited the highest adhesion at 0. 01M KN03 while for B. subtilis is at 0. 0001M KNO3. At different pH conditions, it is observed that the adhesion of both bacteria increased until pH 5 and decreased thereafter. This study has its potential application in the bioflotation of Philippine gold ores.

Zeta potential bacterial adhesion E. colis B. subtilis gold substrate DLVO

Jose Carlo M. Viguilla Windell L.Rivera Herman D. Mendoza

Department of Mining, Metallurgical, and Materials Engineering College of Engineering, University of Molecular Protozoology Laboratory, Natural Sciences Research Institute and Institute of Biology, Col

国际会议

The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)

长沙

英文

452-459

2011-09-18(万方平台首次上网日期,不代表论文的发表时间)