Isolation and identification of four strains of Leptospirillum ferriphilum from four acid mine drainage sites and their roles in bioleaching of pyrite

摘要：

Abstract; In this study, four bacterial strains - DWT, SLS, YTW and ZBY were isolated from four different acid mine drainage ( AMD) sites using serial dilution method. Their morphological and physiological characteristics, phylogenetic relationship and ability in bioleaching of pyrite were studied respectively. Physiological examination showed that the optimal temperature for the growth of strains DWT, SLS, YTW and ZBY were 351, 351, 351 , 40° C , respectively; and the optimal pH for their growth were 1. 5 , 1. 8 , 2. 1 and 1.8, respectively. The results of phylogenetic analyses based on 16S rRNA and gyrB gene suggested that all the isolated strains should be affiliated with the species of Leptospirillum ferriphilum. Bioleaching experiment showed that all the strains could oxidize pyrite efficiently, and the iron extraction rates achieved a high level ranging from 80％ to 93％ after 18 days.

关键词： Leptospirillum ferriphilum isolation rep-PCR 16S rRNA and gyrB gene bioleaching

作者: Yi Liu Huaqun Yin Yao Liu Qiaoyi Tan Yili Liang Xueduan Liu

作者单位: Key Laboratory of Biometallurgy of Ministry of Education,Central South University, 410083, Changsha, China School of Minerals Processing and Bioengineering,Central South University, 410083, Changsha, China

会议类型: 国际会议

会议名称: The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)

会议地点: 长沙

会议语种:英文

页码: 651-655

在线出版日期: 2011-09-18（万方平台首次上网日期，不代表论文的发表时间）