Bioleaching of copper sulphide sludge
The aim of this work was to research potentially novel extreme thermophilic acidophiles populated in RTB Bor copper ores, chalcopyrite concentrates and mine waters which are able to oxidise iron with similar or enhanced bioleaching capability compared with mesophiles/moderately thermophilic and a particular focus on bioleaching of copper sulphide minerals. The culture was identified by Quantitative Polymerase Chain Reaction ( Q - PCR analyse) for measuring bacterial abundance in environmental samples. The relative proportions of the cultures present in analysed mine samples did not vary much. It was shown that the dominant organism was Acidianus sp. , with Metallosphaera sp. present in lower numbers. For this purpose selected sample of mine water was collected from water generated within V. Krivelj mine, and enriched in a basal nutrinet medium (pH 1. 7) and in medium containing yeast extract. Enriched culture in basal nutrient medium and yeast extract were incubated at temperature 701. Detected acidophilic thermophiles culture were morphologically similar and consisted of round bacteria irregularly shaped. Following isolation, incubation and adaptation, the mixed culture enriched in basal nutient medium was used in a batch test to check the ability of the thermophiles to oxidise a copper sulphide sludge, comprising of covellite (98 ％ ). Bioleaching experiments showed that the mixed culture was capable of rapidly oxidation a CuS in copper sulphide sludge, up to 98％ Cu release in 84 h. The performance of the batch test was monitored in terms of pH, redox potential, dissolved copper, copper efficiency, elemental sulphur and sulphides. Incubation and growth of thermophiles were examined microscopically.
enrichment bacteria covellite thermophiles biooxidation
Milena Cvetkovska Vladimir Cvetkovski Milovan Vukovic
University of Belgrade, Faculty of Chemistry, Department for Biochemistry,Studentski Trg 15, 11000, Mining and Metallurgy Institute Bor, Zeleni Bulevar35 19210 Bor, Serbia University of Belgrade, Department for Metallurgy,Vojska Jugoslavije, 19210 Bor, Serbia
国际会议
The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)
长沙
英文
764-769
2011-09-18（万方平台首次上网日期，不代表论文的发表时间）