Microbially induced flotation of galena and quartz from pyrite
Cells and metabolic products of B. subtilis, was used in flocculation, flotation and adsorption studies of sulphide such as pyrite, galena and oxide minerals quartz. Selective affinity of the bacterial cells towards various mineral surfaces was observed through adsorption studies. Bacterial byproduct like extracellular bacterial protein ( EP) was isolated from B. subtilis. The protein profile of the EP of bacterial cells grown in presence of minerals was also studied. EBP was separated through ammonium sulphate precipitation method and characterized through SDS-PAGE. Protein so obtained exhibited varying surface adsorption capacity towards sulphide and oxide minerals. When bacterial cells were grown in the presence of the minerals, mineral-specific proteins were expressed depending on the mineral to which cells were exposed during growth. Protein fractions having significant surface affinity towards different minerals were isolated and their role in mineral separation processes demonstrated. This study has demonstrated the utility and amenability of microbially-induced mineral beneficiation through the use of bacterially generated proteins. Selective separation of pyrite from galena and quartz was achieved through microbiologically induced flotation in presence of Bacillus subtilis. B. subtilis functions as a depressant for pyrite while it promotes the flotation of galena and quartz.
Bacillus subtilis extracellular proteins flotation sulfide minerals
H. Sarvamangala K. A. Natarajan
Department of Materials Engineering, Indian Institute of Science, Bangalore, 560012, India
国际会议
The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)
长沙
英文
855-860
2011-09-18（万方平台首次上网日期，不代表论文的发表时间）