Succession of AMD bacterial communities under high Fe3+ concentration
In acid mine drainage ( AMD) , the bacteria rapidly oxidize ferrous ions to produce a large amount of ferric ions in the surrounding AMD environment. However, the importance of ferric ions in the bacterial metabolism and succession of microbial community structure has attracted little attention. The aim of the investigation was to study the succession of bacterial communities under various Fe3 + concentrations by Amplified Ribosomal DNA Restriction Analysis (ARDRA) method. The result showed that the growth of acidophiles inhabited in the AMD environments was remarkably influenced by Fe + concentration. A total of 8 operational taxonomic units ( OTUs) were recovered in two samples (JX and FS) , when they were cultured in media with increased Fe3+ concentration. These OTUs were affiliated to a-Proteobacteria ( mainly Acidiphilium sp. ) , -y-Proteobacteria ( mainly A. ferrooxidans) , Nitrospira ( mainly Leptospirillum sp. ecies) , and Actinobacteria ( Ferrimicrobium acidophilum). The succession of bacterial community indicated the existence of a competitive relationship between A. ferrooxidans and Leptospirillum-like bacteria. A. ferrooxidans grew initially in the media of high Fe2+ but low Fe3 + concentrations. The growth rate of A. ferrooxidans was found significantly inhibited at high Fe3+ concentration. For Leptospirillum-like species, the growth rate was less sensitive to Fe + and their iron-oxidizing ability was not significantly affected. When the sample FS was cultured in the medium with 12. 5 g/L of ferric ions, a bacterium which had 99％ phylogenetic similarity to Ferrimicrobium Acidiphilum became the most predominant species.
AMD microbial community succession ferric ion amplified ribosomal DNA restriction analysis (ARDRA)
Mengxue Diao Kai Liu Minxi Wan Anh V. Nguyen Yu Yang
School of Chemical Engineering, The University of Queensland, Brisbane, 4072, Queensland, Australia School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha,China School of Minerals Processing and Bioengineering, Central South University, 410083, Changsha,China K
国际会议
The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)
长沙
英文
952-959
2011-09-18（万方平台首次上网日期，不代表论文的发表时间）