A novel acidophilic y-proteobacterium for bioleaching
The novel acidophilic iron-oxidizing -yproteobacterium SPIII/3 was isolated from an open cast mining area in the Iberian Pyrite Belt near to La Esperanza in Murcia, Spain. Attachment to and leaching-behaviour of pyrite grains of isolate SPIII/3 and its closest relative Acidithiobacillus ferrooxidans DSM 2392 were investigated. Attachment assays of cells of SPIII/3 to pyrite (grain size 50 100 (xm) revealed 60％ attachment within the first 20 min to and a total attachment of approx. 80％ of the cells after 4 h, while cells of DSM 2392 attached mainly within the first 20 min and reached approx. 40％ . After 2 h equilibrium was reached at approx. 50％ attachment. The pyrite dissolution of SPIII/3 as determined by total dissolved iron after 21 days was 38 mM. Corresponding to the iron dissolution, 75 mM sulfate was detected. Acidithiobacillus ferrooxidans DSM 2392 failed to leach pyrite. Additionally, biofilm formation of cells of strain SPIII/3 on pyrite coupons was visualized using a combination of epifluorescence- and atomic force-microscopy. Microscopic studies revealed the formation of a cell monolayer and cluster of several cells on the pyrite surface.
bioleaching bacteria biofilm formation attachment AFM EFM
Christian Thyssen Mario Vera Claudia Janosch Wolfgang Sand
Aquatic Biotechnology, Biofilm Centre,University of Duisburg Essen,Universitatsstr. 5, 45141 Essen, Germany
国际会议
The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)
长沙
英文
979-981
2011-09-18（万方平台首次上网日期，不代表论文的发表时间）