A lusatian open cast lignite mine—A source for new iron-oxidizing microorganisms?
Acid mine drainage ( AMD) with high sulphate concentrations triggered a microbiological study in an open lignite mining area in Eastern Germany as a basis for a development of sustainable countermeasures 1 . In 2006 and 2008 sampling campaigns were performed. In 2006 the DNA of Acidithiobacillus ferrooxidans, A. thiooxidans, and Leptospirillum ferrooxidans was detected by PCR experiments. The Acidithiobacillus species were equally distributed in the overburden. In the 2008 campaign four sand samples were taken ten meters above the lignite, two samples from aquifer material, and four samples visibly striking by brown colour. All samples allowed DNA extraction. Bacterial 16S rRNA genes and in the two samples from aquifer material archaeal 16S rRNA genes were amplified. Specific amplificates for A. ferrooxidans and A. thiooxidans were achieved from nine or seven samples, respectively. In one aquifer material sample an amplificate specific for Leptospirillum spp. was detected. Pure cultures at 28 °C were achieved for A. ferrooxidans, A. thiooxidans, L. ferrooxidans, L. ferriphilium and Acidiphilium acidophilium. Surprisingly, Ferrimicrobium acidiphilium, a bacterium described in 2009 for the first time 2 , also occurred in aquifer material from the fresh overburden. Cultivation experiments for iron- and sulphur-oxidizing microorganisms at 28t with the 2008 samples revealed growth in all cases. Furthermore, by cultivation at 45X1 iron-oxidizing microorganisms were detected in two aquifer samples from 2008, whereas in 2006 no such activity was traceable. One pure culture was achieved and identified by sequencing of the 16S rRNA genes as Sulfobacillus acidophilus with 99 percent identity. The detection of F. acidiphilium clearly encourages the search for other unknown leaching microorganisms in these acid mine drainage areas. To abate AMD first experiments with tensides like SDS are performed. A serious reduction of sulphate efflux from sand samples with up to 1 ％ of pyrite as source of acidification and sulphate was achieved.
acid mine drainage bioleaching lignite mining 16S rRNA gene
Hans-Michael Siebert Tobias Uhlig Silvia Hondke Wolfgang Sand K. Peter Stahmann
Bio-, Chemie-und Verfahrenstechnik, Hochschule Lausitz (FH),Gropenhainer Str. 57, D-01968 Senftenber Aquatische Biotechnologie, Biofilm Centre, Fakultat fur Chemie,Universitat Duisburg-Essen, Universit Aquatische Biotechnologie, Biofilm Centre, Fakultat fur Chemie,Universitat Duisburg-Essen, Universit
国际会议
The 19th International Biohydrometallurgy Symposium(第19届国际生物湿法冶金大会 IBS2011)
长沙
英文
1002-1006
2011-09-18（万方平台首次上网日期，不代表论文的发表时间）