Analysis and Handling of Bio-nanoparticles and Intact Viruses using Electrostatic Aerosol Mobility
The utilization of differential mobility analysis for the separation of singly charged particles by their electrophoretic mobility diameter at atmospheric pressure together an appropriate detection instrument allows measurement of the individual electrostatically separated singly charged nanoparticles (ions). A direct relationship between molecular mass and equivalent diameter was obtained yielding a possibility of determination of molecular masses of high molecular weight proteins and non-covalent protein complexes and observation of influence of different environmental conditions on the stability of the selected protein complexes. The study of human rhinoviruses has demonstrated the capability of the technique to analyze the intact infectious human-pathogenic viruses and corresponding antibodies along with the monitoring of the dissociation of these supra-molecular structures by means of heat.
Nanoparticles Bio-macromolecules Virus detection Protein stability PDMA GEMMA
Peter Kallinger Anne Maiβer Günter Allmaier Wladyslaw W. Szymanski
University of Vienna,Faculty of Physics,Boltzmanngasse 5,A-1090 Vienna,Austria Vienna University of Technology,Institute of Chemical Technologies and Analytics,Getreidemarkt 9,A-1
国际会议
西安
英文
168-172
2011-08-17（万方平台首次上网日期，不代表论文的发表时间）