Comparative research on two kinds of amperometric immunosensor for detection of carbofuran residues
In this study, two kinds of novel label-free amperometric immnnosensor for the direct detection of carbofuran was developed using layer-by-layer assembly and sol-gel technology, respectively. In one hand anti-carbofuran monoclonal antibodies were immobilized on the gold electrode (GE) surface which was modified with multilayers of L-cysteinewas, gold nanoparticles (nano-Au) and horseradish peroxidase (HRP) through layer-by-layer assembly technology, in the other hand, anti-carbofuran monoclonal antibodies were immobilized on the surface of the glassy carbon electrode (GCE) based -on sol-gel technology to prepare non-labeled electrochemical immunosensor for the detection of carbofuran. The conformational properties of the immunosensor were characterized using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and a current response curve. Effects of experimental variables, such as concentration of antibody solution, pH of working buffer and incubation time, were studied in detail for optimization of analytical performance. Under optimal conditions, the current response was proportional to the concentration of carbofuran.
amperometric immunosensor comparative research layer-by-layer assembly sol-gel technology carbofuran detection
Shuyuan Du Xia Sun Xiangyou Wang
Department of Agriculture and Food Engineering of Shandong University of Technology Shandong Province, P.R. China
国际会议
山东淄博
英文
651-654
2011-05-27（万方平台首次上网日期，不代表论文的发表时间）