Studies on the Cellulase Production Using Corncob Residue from Xylose Manufacture by Trichoderma sp.B-8 and the Characterization of the Cellulase

摘要：

To improve enzymatic hydrolytic efficiency and reduce production cost, the cellulase production by Trichoderma sp. B-8, isolated from corncob residue from xylose manufacture^ was investigated, using corncob reside as main carbon source. The optimal carbon and nitrogen sources used in the fermentation medium were as follows: corncob residues 2.5％, wheat bran 0.75％, peptone 0.5％ and corn steep liquor 2.0％. The optimal hydrolysis temperature and.pH of the cellulase were 45*C and 5.0, respectively. The activities of FPAase and CMCase of the crude cellulase liquor determined at the optimal hydrolysis temperature and pH reached 3.32IU/ml and 25.7ILI/ml, respectively. In addition, the most important, the glucose production by saccharifying corncob residue using crude cellulase was as high as 41.0g/L on the 10％ (W7V) corncob residues base.

关键词： corncob residue from xylose manufacture cellulase enzymatic properties enzymatic hydrolysis

作者: Xiaomin Fang Weichi Liu Huiqin Shi Jianlu Ma Zhiwen Jiang Guifu Dai Jian Wu Xiaodong Qin Songyang Lin Yinlong Song

作者单位: Department of Bioengineering, Zhengzhou University Zhengzhou, China

会议类型: 国际会议

会议名称: 2011 International Conference on Agricultural Engineering New Technology & Taishan Academic Forum(2011农业工程新技术国际学术会议暨泰山学术论坛 ICAE2011&TSAF)

会议地点: 山东淄博

会议语种:英文

页码: 684-687

在线出版日期: 2011-05-27（万方平台首次上网日期，不代表论文的发表时间）