Optimum Parameters of Preparation of Epidermal Cell Protoplasts within the Allium Cepa L

摘要：

Objective to optimize the- isolation condition of Epidermal Cell Protoplasts within the Allium cepa L. Methods According, to orthogonal experiment design L25 (56).of enzyme, osmotic pressure stabilizer(DMannitol), pH, enzymolysis time, we got the optimal isolation condition by the number of protoplasts. Results The optimal isolation condition is showed in results: enzymolysis time 2h, pH 5.5, D-Mannitol 0.6 mol/L, 1.5％ cellulose and 0.1％ pectolyase and 0.1％ BSA, temperature 28 ℃.It also means that optimal isolation condition is D5B3QA2. Conclusion The optimal isolation condition is viable. .

关键词： Allium cepa L Protoplast Preparation Orthogonal experiment

作者: Hu Jing Ma Wanzheng Mao Hanpin Han Lvhua

作者单位: Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education & Jiangsu Prov Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education & Jiangsu Prov

会议类型: 国际会议

会议名称: 2011 International Conference on Agricultural Engineering New Technology & Taishan Academic Forum(2011农业工程新技术国际学术会议暨泰山学术论坛 ICAE2011&TSAF)

会议地点: 山东淄博

会议语种:英文

页码: 922-924

在线出版日期: 2011-05-27（万方平台首次上网日期，不代表论文的发表时间）