Synthesis of Artificial Antigen and studied on influence factors of ELISA for Chlorothalonil residue
In this work, the chlorothalonil derivative (i.e. hapten) was synthesized. The chlorothalonil artificial antigens were prepared via the hapten conjugated with carrier proteins by activated-ester and mixed-anhydride method, respectively. The antiserum were obtained through immunizing the New Zealand white rabbits with the immune antigen, and the appropriate concentrations of coating antigen and antibody of ELISA were determined with chessboard format. Meanwhile, the influence factors df washing processes were also investigated, which evaluated by the value of OD490. The results showed that theartificial antigens of chlorothalonil were successfully synthesized, and the antiserum titer was about l:1.0xl04. The detection limit (IC20) of ELISA was 0.0123ng/mL. The recoveries of ELISA were from 84.0％ to 89.8％, Coefficient of variation ranged from 3.0％ . to 7.6％. And all the Cross-reactivity (CR) with chlorothalonil analogous structure compounds were less than 0.01％.
Chlorothalonil Artificial antigen ELISA Polyclonal Antibody Washing plate
Guo Nai-fei Ji Shu-juan Yu Ji-cheng
College of Food Science, Shenyang Agricultural University, Shenyang, China College of Life Science,D College of Food Science, Shenyang Agricultural University, Shenyang, China College of Life Science,Dalian Nationalities University,Dalian, China
国际会议
山东淄博
英文
1018-1021
2011-05-27（万方平台首次上网日期，不代表论文的发表时间）