Determination of free amino acids and taurine in Sinonova- culaconstricta with 2,4-dinitrochlorobenzene Precolumn Derivatization

摘要：

A method was established to determination the 18 kinds of free amino acids and the taurine in Sinonovacula constricta by HPLC with precolumn derivatization with 2,4-dinitrochlorobenzene as a derivatization agent,A Kormat uinversil C18 column was used with gradient elution at the detection wavelength of 360 nm.There is a good linear realationship between the peak areas and concentration in the range of 1~50 u.mol/L of the 18 kinds of free amino acids and taurine.The average recovery is 93.7％—110.3％,The detection limit is 0.36—0.86 umol/L and RSD is 1.01 ％~4.46％.

关键词： taurine free amino acids precolumn derivatization HPLC

作者: Wang YueHong Liu HongYin Qi FengSHeng Zhong WeiFang ZhangHui Li Lina

作者单位: Ocean college Hebei agriculturial university,Qin huangdao. China, 066003 Food college Hebei agriculturial university,Baoding, China, 071001

会议类型: 国际会议

会议名称: 2011 International Conference on Agricultural Engineering New Technology & Taishan Academic Forum(2011农业工程新技术国际学术会议暨泰山学术论坛 ICAE2011&TSAF)

会议地点: 山东淄博

会议语种:英文

页码: 1030-1033

在线出版日期: 2011-05-27（万方平台首次上网日期，不代表论文的发表时间）