Purification and Properties of B-Glucosidase,Exo-B-Glucanase and Endo-B-Glucanase from A Commercial Cellulase
The commercial cellulose was purified by means of (NH4)2SO4 depositon, Sephadex G-150 chromatography and DEAE-Sephadexl-50.The specific activity of the pglucosidase increased 93.13 fold and the coefficient of recovery was 6.08％, the molecular mass was 53kDa determined by gel imaging system after SDS-PAGE. The specific activity of the exo-β-glucanase increased 3.86 fold and the coefficient of recovery was 2.02％, the molecular mass was 33kDa determined by gel imaging system after SDSPAGE. The specific activity of the endo-β-glucanase increased 4.07 fold and the coefficient of recovery was 2.6％, the molecular mass was 64kDa determined by gel imaging system after SDS-PAGE. The high filter activity can be obtained by the cocktail mixture in accordance with the specific proportion of the three kinds of enzyme endo-β-glucanase:β-glucosidase : exo—β-glucanase 3: 2: 15. Mg2+-. Fe3+ can effectively activate the three kinds of enzyme at the meantime.
β-glucosidase exo-p-glucanase endo-figlucanase purification Properties
Gong Mei-zhen Lv Yu-cai Tang Jingling Tian Hui Li De-ying Zhao Ru-ming Gong Da Chun
Alan G.Macdiarmid Institute of Renewable Energy China Three Gorges University Yichang, 443002, PR China
国际会议
山东淄博
英文
1100-1103
2011-05-27（万方平台首次上网日期，不代表论文的发表时间）