Study on Establishing 2D-TLC Bioautography of Fast Screening Antineoplastic Active Components

摘要：

In order to construct 2D-TLC bioautography combining PyricuLaria oryza P-2b for fast screening antineoplastic active components,sensitivity of the bioautography to Cisplatin, Fluorouracil, Etoposide , Doxorubicin Hydrochloride and Vinblastina was detected, antineoplastic active components of the Endophytes crude extract were screened by the bioautography and validated by MTT assay method to Hepg2 and A549.The Result showed the bioautography was sensitive to the six antitumor drugs and was identical between P.oryza P-2b TLC bioautography and MTT assay method. The method of 2D-TLC bioautography combining P. Oryzae P-2b was established to quickly screen anti-tumor activity, so as to exploit its application area.

关键词： PyricuLaria oryza P-2b 2D-TLC Bioautography natural product fast screening antineoplastic active components

作者: Qimei Zhao Jia He

作者单位: Forestry School University of Science and Technology Luoyang, China 473003 Food and Bioengineering School,University of Science and Technology Luoyang, China 473003

会议类型: 国际会议

会议名称: 2011 International Conference on Agricultural Engineering New Technology & Taishan Academic Forum(2011农业工程新技术国际学术会议暨泰山学术论坛 ICAE2011&TSAF)

会议地点: 山东淄博

会议语种:英文

页码: 1136-1139

在线出版日期: 2011-05-27（万方平台首次上网日期，不代表论文的发表时间）