会议专题

Study on Establishing 2D-TLC Bioautography of Fast Screening Antineoplastic Active Components

In order to construct 2D-TLC bioautography combining PyricuLaria oryza P-2b for fast screening antineoplastic active components,sensitivity of the bioautography to Cisplatin, Fluorouracil, Etoposide , Doxorubicin Hydrochloride and Vinblastina was detected, antineoplastic active components of the Endophytes crude extract were screened by the bioautography and validated by MTT assay method to Hepg2 and A549.The Result showed the bioautography was sensitive to the six antitumor drugs and was identical between P.oryza P-2b TLC bioautography and MTT assay method. The method of 2D-TLC bioautography combining P. Oryzae P-2b was established to quickly screen anti-tumor activity, so as to exploit its application area.

PyricuLaria oryza P-2b 2D-TLC Bioautography natural product fast screening antineoplastic active components

Qimei Zhao Jia He

Forestry School University of Science and Technology Luoyang, China 473003 Food and Bioengineering School,University of Science and Technology Luoyang, China 473003

国际会议

2011 International Conference on Agricultural Engineering New Technology & Taishan Academic Forum(2011农业工程新技术国际学术会议暨泰山学术论坛 ICAE2011&TSAF)

山东淄博

英文

1136-1139

2011-05-27(万方平台首次上网日期,不代表论文的发表时间)