Study on Establishing 2D-TLC Bioautography of Fast Screening Antineoplastic Active Components
In order to construct 2D-TLC bioautography combining PyricuLaria oryza P-2b for fast screening antineoplastic active components,sensitivity of the bioautography to Cisplatin, Fluorouracil, Etoposide , Doxorubicin Hydrochloride and Vinblastina was detected, antineoplastic active components of the Endophytes crude extract were screened by the bioautography and validated by MTT assay method to Hepg2 and A549.The Result showed the bioautography was sensitive to the six antitumor drugs and was identical between P.oryza P-2b TLC bioautography and MTT assay method. The method of 2D-TLC bioautography combining P. Oryzae P-2b was established to quickly screen anti-tumor activity, so as to exploit its application area.
PyricuLaria oryza P-2b 2D-TLC Bioautography natural product fast screening antineoplastic active components
Qimei Zhao Jia He
Forestry School University of Science and Technology Luoyang, China 473003 Food and Bioengineering School,University of Science and Technology Luoyang, China 473003
国际会议
山东淄博
英文
1136-1139
2011-05-27(万方平台首次上网日期,不代表论文的发表时间)