Robotic Cell Manipulation with Optical Tweezers for Biomechanical Characterization
In this paper, we demonstrate the effectiveness of robotic cell stretching with optical tweezers for biomechanical characterization. Optical traps serve as end-effectors to manipulate micro-beads attached to the cell surface. The dynamics of the cell-bead mixture during cell stretching is investigated for the first time. Based on our previous work, cell stiffness is extracted and biomechanical properties of cells can be characterized. Our study shows that the modeling results agree with the experimental data. Further, the area compressibility moduli of two types of cells, human embryonic stem cells (hESC) and hESC-derived cardiomyocytes (hESC-CM), are characterized. The results indicate that undifferentiated stem cells are much softer than differentiated ones, which provides an important insight into the cell mechanics during hESC differentiation. In summary, this paper successfully demonstrates that the robot-tweezer system can manipulate biological cells effectively to characterize biomechanical properties of living cells.
Youhua Tan Dong Sun Shuk Han Cheng Ronald A. Li
Department of Manufacturing Engineering and Engineering Management,City University of Hong Kong,Hong Department of Biology and Chemistry,City University of Hong Kong,Hong Kong,China Department of Medicine,the University of Hong Kong,Hong Kong,China
国际会议
2011 IEEE International Conference on Robotics and Automation(2011年IEEE世界机器人与自动化大会 ICRA 2011)
上海
英文
4104-4109
2011-05-09（万方平台首次上网日期，不代表论文的发表时间）