Direct Nano-injection Method by Nanoprobe Insertion based on E-SEM Nanorobotic Manipulation under Hybrid Microscope
This paper presents a novel nano-injection method by nanoprobe insertion based on environmental-scanning electron microscope (E-SEM) under hybrid microscope. The hybrid microscope is designed to combine the optical microscope (OM) and E-SEM to realize biological specimen analysis by optical imaging including fluorescent imaging, and nano-scale manipulation by E-SEM imaging. Based on the bio-nano manipulation system, we propose a nano-injection method by nanoprobe insertion. The nanoprobe is designed to have a sharp tip to insert the probe into biological specimen. It is fabricated by focused ion beam (FIB) process. The nano-scale size is important for minimal damage to biological specimen. The Caenorhabditis elegans (C. elegans) is used as a target biological specimen, which is one of the important model organism for diseases analysis. The nanoinjection technique is needed to transport fluorescent materials or specific biological organism into specific cell for in-vivo experiment. The proposed system is considered to be important as future nano-surgery system for life innovation using model organism.
Masahiro Nakajima Takanori Hirano Masaru Kojima Naoki Hisamoto Michio Homma Toshio Fukuda
Center For Micro-nano Mechatronics of Nagoya University,Furo-cho,Chikusa-ku,Nagoya,464-8603 Department of Micro-Nano Systems Engineering of Nagoya University,Furo-cho,Chikusa-ku,Nagoya,464-860 Division of Biological Science of Nagoya University,Furo-cho,Chikusa-ku,Nagoya,464-8602 Center For Micro-nano Mechatronics of Nagoya University,Furo-cho,Chikusa-ku,Nagoya,464-8603 Departme
国际会议
2011 IEEE International Conference on Robotics and Automation(2011年IEEE世界机器人与自动化大会 ICRA 2011)
上海
英文
4139-4144
2011-05-09（万方平台首次上网日期，不代表论文的发表时间）