Browning Inhibition and Regeneration of Red General Fuji Apple
Experiments were conducted with shoot tips and single node explants collected from field growing Red General Fuji apple ( Malus domestica ) trees to study the effects of MS or I QMS medium, pretreatment with low tempreature, ascorbic acid ( VC ) or activated charcoal ( AC ) on browning inhibition. The influences of different concentrations of 6-BA and NAA combinations on shoot proliferation, and 6-BA and IBA combinations on bud regeneration from leaf were also investigated. The results showed that serious browning occurred in this cultivar during the first 2 days in culture. Browning was significantly reduced by pretreatment with 4°Cfor 12 hplus 3.0 g/L AC, which was entirely inhibited by frequent subculture to fresh medium at 2-dayinterval. The optimal medium for shoot proliferation is MS +6-BAI.0 mg/L + NAAO.I mg/L. And the highest shoot regeneration frequency from leaf was 23.33％ with 3.71 shoots per explant, using medium ofMS+ 6-BA 2.0 mg/L+ IBA 0.4 mg/L.
browning Red General Fuji apple activated charcoal regeneration
Duan Yanxin Yuan Yongbing Li Peihuan Liu Chenglian
Qingdao Key Lab of Modern Agriculture Quality and Safety Engineering, Qingdao AgriculturalUniversity Qingdao Key Lab of Modern Agriculture Quality and Safety Engineering, Qingdao Agricultural Universit
国际会议
The 2nd Conference on Horticulture Science and Technology(第二届园艺科学与技术学术会议 CHST 2010)
北京
英文
62-65
2010-12-18（万方平台首次上网日期，不代表论文的发表时间）