Characterization of Phalaenopsis aphrodite Chloroplast Microsatellites
Chloroplast simple-sequence repeats (cpSSRs) were investigated in the Phalaenopsis aphrodite. 61 SSRs were identified. Among them there were 47 (78.3％) mono-, 8 (15.3％) di-. 4 (6.7％) tri-, I (1.7％) tetra-, I (1.7％) penta-, 1(1.7％) hex-nudeotide SSRs, mainly distributed in large single-copy regions (LSC). The SSR frequency was approximately one SSR/2.4kb on an average. The monomuceleotide repeats appeared to be the most abundant. Five cpSSR primer pairs were designed and tested. The result showed that development of genus-septic cpSSR primers based on sequence from the model species was perfectly feasible.
Chloroplast SSR orchid
Zhang Jun-yi
Department of Bioengineering & Biotechnology, Huaqiao University
国际会议
The 2nd Conference on Horticulture Science and Technology(第二届园艺科学与技术学术会议 CHST 2010)
北京
英文
161-165
2010-12-18（万方平台首次上网日期，不代表论文的发表时间）