Cloning and Expression Analysis of WRKY Gene in Eggplant
The WRKY transcription factor protein is famous for its highly conserved amino acid sequence WRKYGQK as well as the novel zinc-finger-like motifs Cys2His2 or Cys2HisCys. The WRKY transcription factor is considered to be widely involved in biotic and abiotic stress responses in plant. In this study, a 702 bp WRKY segment and a 558 bp fi-actin segment were acquired, including WRKY gene expression in eggplant seedlings under the following treatment conditions: salinity (200 mM NaCI), cold(4°C), and abscisic acid (ABA) (100 ftM), and then different expressions of WRKY gene were detected by using RT-qPCR (real-time relative quantification PCR). The results of RT-qPCR analysis revealed that the relative expression of WRKY gene improved 617.47％. 185.83％, 205.30％ after cold, salinity, and ABA treatment respectively, which demonstrated that WRKY gene played a positive regulatory role under different conditions.
eggplant WRKY cloning expression stresses ABA
Qin Song Fukuan Zhao Qingpeng Sun Aizhen Yang
Department of Biotechnology, Beijing University of Agriculture
国际会议
The 2nd Conference on Horticulture Science and Technology(第二届园艺科学与技术学术会议 CHST 2010)
北京
英文
177-180
2010-12-18（万方平台首次上网日期，不代表论文的发表时间）