Methods of Constructing Primary Core Collections Based on the Genotypic Values of Malus Sieversii (Ledeb) Roem
A inlegrative strategy is developed for the establishment of a primary core collection based on predicted genotypic values. A Malus sieversii germplasm collection of 300 accessions with 10 quantitative traits was used to construct core collections. Genotypic values of all quantitative traits of the Malus sieversii collection were unbiasedly predicted based on the mixed linear model approach. The inlegrative strategy is based on stepwise clustering analysis of the predicted genotypic values, selection of accessions from the resulting clusters according to a combination of four hierarchical clustering methods (unweighted pairgroup average method, the single linkage method, the complete linkage method, and Wards method) and three sampling strategies (random sampling, preferred sampling and deviation sampling), optimisation of the sampling proportion, and validation by means of principal component analysis. A core collection with 60 accessions (at 20％ sampling proportion) constructed by the unweighted pair-group average method in combination with the deviation sampling strategy retains the greatest degree of genetic diversities of the initial collection.
Primary core collection Genotypic value Phenotypic value Clustering method Sampling strategy
Zunchun Liu Donge Bao Ruijin Zhou Xuesen Chen
Department of Horticulture, Henan Institute of Science & Technology Department of Horticulture, Shandong Agricultural University
国际会议
The 2nd Conference on Horticulture Science and Technology(第二届园艺科学与技术学术会议 CHST 2010)
北京
英文
196-199
2010-12-18（万方平台首次上网日期，不代表论文的发表时间）