Protective Effects of Emodin and Chrysophanol Isolated from Marine Fungus Aspergillus sp.on Ethanol-Induced Toxicity in HepG2/CYP2E1 Cells
Alcohol-induced liver injury progresses from fatty infiltration followed by a harmful cause of inflammation leading to an irreversible damage. In this study, two compounds (emodin and chrysophanol) isolated from marine fungus Aspergillus sp. Were examined for their protective e.ects against ethanol-induced toxicity in vitro. Ethanol-induced HepG2/CYP2E1 cells were treated with the compounds at various concentrations, and the results showed that there was a dose-dependent decrease of gamma-glutamyl transpeptidase (GGT) activity and increase of glutathione (GSH) in the culture media with an increase in cell viability. Furthermore, the protective e.ects of the compounds were evaluated by protein expression levels of GGT, GSH, and CYP2E1 using Western blot. Among the compounds, emodin addressed to the ethanol-induced cytotoxicity more e.ectively compared to the chrysophanol. It could be suggested that emodin isolated from this genus would be a potential candidate for attenuating ethanol induced liver damage for further industrial applications such as functional food and pharmaceutical developments.
Zhong-Ji Qian Chen Zhang Yong-Xin Li Jae-Young Je Se-Kwon Kim Won-Kyo Jung
Department of Marine Life Science and Marine Life Research and Education Center, Chosun University, Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea Key Laborator Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea School of Food Technology and Nutrition, Chonnam National University, Yeosu 550-749, Republic of Kor
国际会议
青岛
英文
1-7
2010-10-09（万方平台首次上网日期，不代表论文的发表时间）